Exp021scheme.JPG


Objective

To accomplish the Ugi Synthesis and cyclization to a diketopiperazine using 3,4-dihydroxybenzaldehyde, 5-methylfurfurylamine, Boc-Gly-OH, and benzylisocyanide using the protocol described here, with the modification that THF will be used instead of 1,2-dichloroethane in the cyclization step. The target diketopiperazine is not predicted to be active but is a close analog of the product that we wish to make once the catechol aldehyde is obtained.

Procedure

To a 50ml Erlenmeyer flask added methanol (20ml), 3,4-dihydroxybenzaldehyde (67.65mg, 0.49mmol) , 5-methylfurfurylamine (50µl, 0.44mmol) by GC syringe, benzylisocyanide (50µl, 0.41mmol) by GC syringe, and Boc-Gly-OH (87.84mg, 0.50mmol). After vacuuming off methanol, 10%TFA in THF was added and refluxed under nitrogen for 30minutes. An extraction with methylene chloride and water is performed. Vacuum off excess solvent after drying. Perform chromatography for purification if NMR and TLCs warrant.

Results


Characterization of 18B

Viedo of setup with common yellow color. TLC of starting materials vs the Ugi reaction were done when stirring started in 1:1 methylene chloride hexanes.
The order is: amine, acid, aldehyde, benzylisocyanide
Video after 17 hours was taken and unusual red color solution. Second TLC of starting materials vs Ugi in 3:1 methylene chloride hexanes. The order is: acid, amine, benzylisocyanide, aldehyde.
NMR of 18C was taken. JPG is a screen shot from MestRe-C.
HMR screen shot

Characterization of 18C

Video of reflux with 10% TFA in THF
TLC of product after THF but before extraction was against starting materials vs 18B, & 18C.

Characterization of 18D

HMR 9-10 region 6.4-7.6 region 4.9-5.7 region 3.7-4.6 region 0.8-2.6 region

Discussion

The HMR of 18D has many broad peaks and there is a problem with phasing however the amine 5.8-6 region, 2.3-2.5 region and benzene ring 7-8 region are there so continuing to the chromatography step is warranted. Unfortunately due to the chromatotron plate breaking apart during the addition of the solvent the experiment was aborted.
Looking at this review article, the cyclization step can be done at room temperature which could reduce the number of side reactions.

Conclusion

Experiment inconclusive and should be repeated.

Log

2006-07-20

1.17:05] Added aldehyde and acid with methanol to Erlenmeyer flask
2.17:20] Last two components added and stirring started. Sample taken for TLC. Video
3.Performed TLC of starting components with beginning Ugi mixture(18A) in 1:1 methylene chloride hexanes Picture

2006-07-21

4. 13:35] Stopped stirring Video
5. 13:50] Performed TLC in 3:1 methylene chloride hexanes of starting materials vs ugi (18B) Picture
6. Weighed round bottom flask
7. 15:20] Put on rotovap with bath at around 70C
8. 16:00] Took off rotovap

2006-07-24

9. 14:30] Put 18B on high vacuum for an 1.5hours with an average pressure of 1mmHg (18C) weight = 237.7mg
10. Took HMR of 18C

2006-07-26

11. Added 27mL of THF to r.b.f. with 3mL of TFA for reflux
12. 12:07] Put heat on. Set hot plate to low but high temperature accumulates due to malfunction.
13. 12:14] Reflux observed Video
14. 12:46] Round bottom flask taken off of heat
15. 20:00] Extracted with 50mL methylene chloride added to THF solution and 5 x 100mL washings of Distilled water. Water is neon yellow and some of the bottom layer stuck to the separatory funnel as well as was lost in the water layer. pH 6.5 to neutral.
16. Had taken a small sample before extraction for TLC. Amine, acid and benzylisocyanide were done in 1:1 methylene chloride hexanes. Aldehyde was done in 2%methanol in methylenechloride. Pic

2006-07-27

17. Took the washings of water and added it together. Added 50mL methylenechloride and separated without shaking. Took the methylene chloride and washed with 100mL of distilled water. pH was 6.5 to neutral.
18. Combined organic layers were put on rotovap in water bath temp = 50C
19. 18:40] Put remaining methylene chloride on high vacuum
20. 20:40] Took off of vacuum and average pressure was 1.5mmHg.

2006-07-31

21. Added 25mL of dichlormethane to sample to dissolve.
22. Dried with anhydrous magnesium sulfate and filtered off
23. Rotovaped at low temperature about 50C

2006-08-01

24. 12:30] Put sample on high vacuum for 1hr 15 mins with average pressure of 0.8mmHg to get 18D (157mg)
25. Took NMR of vacuumed sample.

2006-08-03

26. Attempted to do chromatotron but the plate fell apart due to channeling that formed from a high flow rate from previous use.

Tags

Benzylisocyanide InChI=1/C8H7N/c1-9-7-8-5-3-2-4-6-8/h2-6H,7H2
3,4-dihydroxybenzaldehyde InChI=1/C7H6O3/c8-4-5-1-2-6(9)7(10)3-5/h1-4,9-10H
Boc-Gly-OH InChI=1/C7H13NO4/c1-7(2,3)12-6(11)8-4-5(9)10/h4H2,1-3H3,(H,8,11)(H,9,10)
5-methylfurfurylamine InChI=1/C6H9NO/c1-5-2-3-6(4-7)8-5/h2-3H,4,7H2,1H3
ugi018: InChI=1/C29H35N3O6/c1-19-10-11-21(14-19)18-32(25(35)17-31-28(37)38-29(2,3)4)26(22-12-13-23(33)24(34)15-22)27(36)30-16-20-8-6-5-7-9-20/h5-13,15,26,33-34H,14,16-18H2,1-4H3,(H,30,36)(H,31,37)
DKP018 InChI=1/C17H18N2O4/c1-10-2-3-11(6-10)9-19-15(22)8-18-17(23)16(19)12-4-5-13(20)14(21)7-12/h2-5,7,16,20-21H,6,8-9H2,1H3,(H,18,23)