Exp075

=Objective= To perform a MALDI MS analysis of the Ugi product 62E.

=Procedure=

//Instrumentation:// All spectra were collected on a Bruker Reflex III MALDI TOFMS operating in reflectron mode. All spectra were collected using the same set of instrument parameters.

//Materials:// PEG 400 calibration standard: 7.36mg/ml in MeOH [|CHCA] matrix: 0.1M stock solution in MeOH [|Dithranol] matrix: 0.1M stock solution in THF LiTFA cationization reagent: 0.0024M stock solution in MeOH Analyte (Ugi product): 1.15mg/ml = 0.023M stock concentration in MeOH

//Methods:// The MALDI sample was prepared by adding 10ul of the analyte solution to 100ul of the matrix solution in a small glass sample vial; the solution was mixed by vortexing briefly. When a cationization reagent (salt) was required, 10ul of the cationization reagent solution was added to the same matrix/analyte mixture. 0.5uL of the sample solution was spotted on the standard Scout-26 stainless steel sample plate and allowed to air-dry (i.e., this is the dried-droplet sample preparation).

=Results=

[|MALDI of Ugi62E product] in CHCA matrix [|MALDI of Ugi62E product] in dithranol matrix [|MALDI of PEG400] in CHCA matrix [|MALDI of PEG400] in dithranol matrix [|MALDI of CHCA] background [|MALDI of dithranol] background

=Discussion=

=Conclusion=

=Tags= 62E[|InChI=1/C25H33N3O7/c1-24(2,3)27-22(30)21(16-9-10-18-19(12-16)34-15-33-18)28(14-17-8-7-11-32-17)20(29)13-26-23(31)35-25(4,5)6/h7-12,21H,13-15H2,1-6H3,(H,26,31)(H,27,30)] [|CHCA] [|InChI=1/C10H7NO3/c11-6-8(10(13)14)5-7-1-3-9(12)4-2-7/h1-5,12H,(H,13,14)/b8-5+] [|Dithranol] [|InChI=1/C14H10O3/c15-10-5-1-3-8-7-9-4-2-6-11(16)13(9)14(17)12(8)10/h1-6,15-16H,7H2] LiTFA [|InChI=1/C2HF3O2.Li/c3-2(4,5)1(6)7;/h(H,6,7);/q;+1/p-1]

= =

media type="custom" key="44592"