EXPLAN002

=Objective= To monitor the reaction of the imine formed with an acid (amino or carboxylic acid).

=Steps=
 * 1) Make up separate 1 mL of 1M solutions of any aromatic aldehyde, any amine and any acid in MeOH-d4.
 * 2) Take HMRs and CMRs of the aldehyde, acid and amine separately.
 * 3) Combine the aldehyde and amine solutions into a 1 dram vial and shake vigorously then transfer to an NMR tube.
 * 4) Take HMR at 5, 10 and 20 minutes after mixing.
 * 5) Take CMR at 25 min after mixing.
 * 6) Take HMR at 40 min after mixing.
 * 7) Take CMR at 45 min after mixing.
 * 8) Take HMR at 80 mins after mixing.
 * 9) Take CMR at 85 mins after mixing.
 * 10) Continue to take NMRs after interval doubling until no more change is observed.
 * 11) Remove water with MgSO4, or any other means.
 * 12) Filter if nesessary depending on technique used.
 * 13) Add prepared acid solution to imine solution and shake.
 * 14) Take HMR at 5, 10 and 20 minutes after mixing.
 * 15) Take CMR at 25 min after mixing.
 * 16) Take HMR at 40 min after mixing.
 * 17) Take CMR at 45 min after mixing.
 * 18) Take HMR at 80 mins after mixing.
 * 19) Take CMR at 85 mins after mixing.
 * 20) Continue to take NMRs after interval doubling until no more change is observed.

=Vatiations= Other NMR solvents that we have seen to work in our experiments or other papers (eg CDCl3, DMSO, etc). All amine and aromatic aldehyde combinations.