mettlertrial

[|MiniMapper facilities list.pdf]
 * Company:** [|Mettler Toledo]
 * Date for trial:** Thursday May 29, 2008
 * Location:** Drexel Bradley lab in 511 Disque
 * Modules:** liquid handler - 2 x miniblock 48 ([|miniblock brochure])
 * Software:**
 * Workflow Specs:** **//link to XML schema//**

[|Chemicals available] (at least 25g) different from Library3 starting materials.

#1 Optimize reaction known to work: EXP189
Final concentration of reagents in methanol 0.5M t-butyl isonitrile boc-gly benzaldehyde furfurylamine
 * parallel runs:** 48
 * Based on:** EXP099 where precipitate was observed within hours

Make up 2M methanol solutions of starting materials Total volume should be 2 mL concentration - 0.5M, 0.25M and 0.1M reagent excess - 1.0 and 1.2 equivalents solvent (for 0.25 and 0.1 conc only): ethanol, acetonitrile, THF
 * Planned workflow (48 experiments total):**
 * Parameters to modify:**

Experiments are listed in this [|spreadsheet]

#2 check for new precipitates: EXP190
[|MettlerTrial2 spreadsheet] that anyone can edit list of available starting materials: Library3
 * parallel runs:** 48

#3 crowdsourcing trial
[|MettlerTrial3 spreadsheet] that anyone can edit list of available starting materials: Library3
 * parallel runs:** 96

Factorial Design of Experiments (K Owens comments)
I think a Norm Abram "rule for wikiposting" should be "think twice, post once". I neglected the fact that there are 4 different reagents (not one) and that if amounts of the reagents change then the ratios (excess) of one reagent over another are varied that way (the experimental factors are not independent). I'll work on this again and repost.