Exp245

=Objective= To synthesize the top 20 Ugi products from the precipitate prediction library run dated 29th July, these also correspond to the [|DoUgi] request numbers 112-131

=Procedure= =Results= [|UCExp245-i] [|UCExp245-ii] [|UCExp245-iii] [|UCExp245-iv] [|UCExp245-v]
 * Pictures**

HNMR [|UCExp245-2A]** Spreadsheet- Ugi reaction progress calculator
 * [|UCExp245-1A]

[|Spreadsheet UCExp245] media type="custom" key="4220071"

=Discussion= None of the reactions formed any ppts. These results, incorporated in the [|CombiUgiResults] sheets should enhance the prediction capability of the model.

=Conclusion= Solid Ugi products were not obtained for any of the runs.

=Log= = = 13:10 Weighed out a piperonal (1.501g, 10mmol), 3,4-methylenedioxyphenylacetic acid (0.901g, 5mmol) and N-Cbz-L-proline (0.499g, 2mmol). 13:40 Made-up a 5M solution of piperonal (1.501g, 10mmol) in methanol in a 2mL volumetric flask. 13:55 Made-up a 1M solution of  3,4-methylenedioxyphenylacetic acid (0.901g, 5mmol) in methanol in a 1mL volumetric flask. 14:20 Made-up a 2M solution of N-Cbz-Lproline (0.499g, 2mmol) in methanol in a 1mL volumetric flask. 14:45 Labeled 40 half dram vials **1-40** 15:40 Pipetted out methanol into vials which needed them see [|Spreadsheet UCExp245] column labeled '"Extra solvent added (uL)" 16:00 Started pipetting out amine (0.1mmol) to each vial---see spreadsheet for details. 16:20 Pipetted 5M piperonal solution (20uL, 0.1mmol) 16:35 Pipetted out the carboxylic acid solution (0.1mmol) to each vial-see spreadsheet for details. 17:00 Pipetted out isocyanide (0.1mmol) to the vials. 17:30 Vortexed the vials for 10s each. 17:50 Obtained pictures of the vials sitting in well plates. [**UCExp245-i, UCExp245-ii, UCExp245-iii**] 18:00 None of the reactions have precipitated so far. Obtained pictures of the vials. [**UCExp245-iv, UCExp245-v**] 19:35 The reaction mixtures from vials 1 abd 2 were transferred in to clean NMR tubes. CDCl3 (~700uL) was added to them. 20:00 Obtained HNMRs of the reaction mixtures from vials 1 and 2, these are recorded in the results section as **UCExp245-1A and UCExp245-2A**
 * 2009-08-18**
 * 2009-08-19**