EXPLAN005

=Objective= To carry out Ugi reactions as quickly and as reproducibly as possible on small scales for products that precipitate out of methanol.

=Steps=
 * 1) Weigh a 2 ml Eppendorf tube and write weight on the tube
 * 2) Fill with 500 microliters of methanol
 * 3) Add 500 micromoles of amine, aldehyde, acid and isonitrile in that order. After each addition, vortex for 15 seconds and confirm that a homogeneous solution has been obtained. If a clear solution is not obtained at any point abort.
 * 4) Leave tube at room temperature for 24 hours.
 * 5) Note the presence of a precipitate then vortex for 15 seconds.
 * 6) Centrifuge at x RPM for 60 seconds then discard supernatant.
 * 7) Add 500 microliters of methanol, vortex for 15 seconds then centrifuge at x RPM for 60 seconds.
 * 8) Repeat the washing procedure for a total of 3 washes.
 * 9) Put open tube under high vac for 15 minutes.
 * 10) Record weight and obtain H and C NMR of entire sample in CDCl3.
 * 11) Submit small sample for MS analysis.

=Variations=
 * 1) Amine and Aldehyde can be mixed and left for 3 hours before addition of acid and isonitrile to see if it makes any difference.
 * 2) One dram vials can be used instead of Eppendorf tubes.
 * 3) Use vortexing or sonication to induce precipitation.