Exp123

=Objective= To synthesize Ugi adducts following the EXPLAN005. The compounds will contribute toward a library for enoyl reductase inhibitors. =Procedure= A Ugi reaction was carried out in a 1.5 ml Eppendorf tube. In the tube, methanol (500uL), furfuylamine (0.25mmol, 22.1uL), o-vanillin (0.25mmol, 38mg), Boc-Tryptophan (0.25mmol, 76.1mg), and n-butyl isocyanide (0.25mmol, 26.1uL) were added in that order. After each addition the solution was vortexed for 15s and made sure a homogeneous solution was obtained. The Eppendorf tube was left at room temperature.

=Results=

=Discussion= There was no precipitate observed in the solution after sitting overnight.

=Conclusion= A Ugi product was not obtained.

=Log=

2007-08-15
13:30 Weighed Eppendorf tube (0.8195g) and labeled 13:35 Weighed out o-vanillin (38mg) 13:45 Weighed out Boc-Tryptophan (76.1mg) 13:50 Filled tube with methanol (500uL), solution was clear 13:55 Added furfurylamine (22.1uL) to tube 13:58 Mechanically mixed on vortex for 15 seconds 14:00 Added o-vanillin (38mg) to tube, solution turned into a bright yellow color 14:02 Mechanically mixed on vortex for 15 seconds 14:05 Added Boc-Tryptophan (76.1mg) to solution 14:07 Mechanically mixed on vortex for 15 seconds 14:15 Added n-butyl isocyanide (26.1uL) to solution 14:17 Mechanically mixed on vortex for 15s, everything was completely dissolved 14:20 Let solution sit undisturbed in the 1.5mL Eppendorf tube

2007-08-16
12:30 Observed no precipitate, solution was clear